Protein Marker PM2500 ExcelBand™ 3-color Regular Range Protein Ladder, 2x 250 uL
Protein Marker PM2500 SMOBIO ExcelBand 3-color Regular Range Protein Marker is a ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 10 to 180 kDa in Tris-Glycine Buffer (9 to 170 kDa in Bis-Tris (MOPS) buffer and 10 to 180 kDa Bis-Tris (MES) buffer).
Proteins in Protein Marker PM2500 are covalently coupled with a blue chromophore except for two reference bands (one green 25 kDa and one red 75 kDa band respectively) when separated on SDS-PAGE (Tris-Glycine buffer).
SMOBIO ExcelBand™ 3-color Regular Range Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol).
Under suggested conditions, Protein Marker PM2500 ExcelBan 3-color Regular Range Protein Marker resolves 10 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane.
4°C for 3 months
-20°C for 24 months
Migration pattern of PM2500 in Tris Glycine Buffer
Migration pattern of PM2500 in Bis Tris Buffer
If you are using harsh buffers in Western Blotting application we have an enhanced version Protein Marker PM2510 in which more prestained proteins give much sharper bands.