FluoroDye™ DNA Fluorescent Loading Dye (Green, 6x), DL5000, 1000ul/Vial
DL5000 SMOBIO FluoroDye DNA Fluorescent Loading Dye is a ready-to-use 6× DNA loading dye designed for fast qualitative electrophoresis analysis.
Containing sensitive fluorescent dye with high specific affinity towards double stranded DNA (dsDNA), the FluoroDye Fluorescent DNA Loading Dye DL5000 has negligible background and renders de-staining process unnecessary. The FluoroDye DNA Fluorescent Loading Dye allows the user to immediately visualize electrophoresis result upon completion or to monitor the electrophoresis in real time.
FluoroDye DNA Fluorescent Loading Dye DL5000 is compatible with both the conventional UV gel-illuminating system as well as the less harmful long wavelength blue light illumination system. FluoroDye emission as bound to dsDNA is 522 nm, while its excitation peaks are at 270, 370 and 497 nm (Fig. 1).
Fig. 1. DL5000 FluoroDye Flurescent Loading Dye (Green 6×) emission as bound to dsDNA is 522 nm while its excitation peaks are at 270, 370 and 497 nm.
DL5000 FluoroDye DNA Fluorescent Loading Dye is stored in 6× concentration in 60% glycerol and buffered with Tris-HCl and EDTA, containing Bromophenol blue, Xylene cyanol FF and Orange G as tracking dyes.
Fig. 2. The FluoroDye DNA Fluorescent Loading Dye is capable of detecting dsDNA fragments down to 0.3 ng in DNA markers (SMOBiO DM3100) in 2× serial dilution.
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