SMOBIO Nucleic Acid Gel Staining Dye

$75.00

  • SMOBIO Nucleic Acid Gel Stain Dye
  • FluoroVue Fluorescent Staining Dye
  • High Staining Sensitivity
  • Stain both DNA and RNA
  • 10,000x High Concentration
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NS1000 FluoroVue™ Nucleic Acid Gel Stain (10,000X), 500ul/Vial

SMOBIO FluoroVue™ Nucleic Acid Gel Staining Dye NS1000 (10,000X) is specially designed for in-gel use and is a safer replacement for conventional Ethidium bromide (EtBr), which poses a significant health and safety hazard for its user.

SMOBIO FluoroVue Nucleic Acid Strain NS1000 is a fluorescent stain dye which offers high sensitivity detection of double-stranded or single-stranded DNA and RNA in a convenient manner. The FluoroVue™ Nucleic Acid Gel Stain offers high sensitivity (Table 1 and Fig. 2) that is several times greater than EtBr.

Required Dye

Sensitivity

Convenience

In-Gel Staining

4 ul

0.14 ng

Very Good

Staining During Electrophoresis

30 ul

0.56 ng

Very Good

Post Stain

10 ul

0.56 ng

Good

Table 1. Different staining methods for using the FluoroVue™ Nucleic Acid Gel Stain

Fig. 1. The emission and exitation spectrum of FluoroVue™ Nucleic Acid Gel Stain (NS1000)

For detailed protocols of different staining methods: please see pages 5~7. We recommend using an in-gel staining method for optimal effect. 1 With a mini horizontal gel electrophoresis system: Combine 40 ml of agarose gel with 300 ml running buffer. The regular post staining buffer volume is 100 ml. 2 Sensitivity is evaluated according to the 4 kb band of DM3100.

Single Strand nucleic acid RNA Staining Dye
Fig. 2. The FluoroVue™ Nucleic Acid Gel Stain shows a green-yellow fluorescence under blue light excitation. The sensitivity of NS1000 is about 0.14 ng (arrow) for a 4 kb fragment.

FluoroVue™ Nucleic Acid Gel Stain is compatible with both conventional ultra violet gel-illumination systems as well as the harmless long wave length blue light illumination systems, like B-BOX™. When bound to nucleic acids, the FluoroVue™ Nucleic Acid Gel Stain has a fluorescent excitation maxima of ~250 and ~482 nm, and an emission maximum of ~509 nm (Fig. 2). Therefore, it can replace EtBr without the need for changing existing lab imaging systems.

Contents
Proprietary dye in a 10,000X concentration.

Caution
Dispose of the stain in accordance to local rules and regulations. The fluorescent staining dye stock solution should be handled with particular caution because the solvent is known to facilitate the entry of organic molecules into tissues. There is no data that addresses the mutagenicity or toxicity of the fluorescent dye in humans. However, the fluorescent dye binds to nucleic acids, thus it should be recognized as a potential mutagen and used with appropriate care.

Features

  • Excellent for in-gel staining
  • Sensitivity: 0.14 ng (DNA) or 1 ng (total RNA)
  • A safer alternative to EtBr
  • Compatibility: suitable to blue or UV light
  • Increased cloning efficiency (blue light)

Working Reagent Preparation
1:10,000 dilution in TAE or TBE buffered agarose
Please see page 5, protocol 1.

Storage 
Protected from light
4°C for 12 months
-20°C for 24 months

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SMOBIO

SMOBIO, A Taiwan Biotech company, manufactures DNA Ladders, DNA Staining Dyes, Protein markers and protein staining dyes, regular PCR and quantitative real time PCR, qPCR related products, Cloning, reverse transcriptase, precast Gel, competent cell and small equipment LED Illuminator, Electrophoresis, etc.