ExcelBand™ 3-color Extra Range Protein Marker PM2800, 10 ~ 310 kDa, 2x 250 µl
SMOBIO Protein Marker PM2800, ExcelBand 3-color Extra Range Protein Marker, is a ready-to-use tri-color protein standard with 13 pre-stained proteins covering an extra range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer (9 to 290 kDa in Bis-Tris (MOPS) buffer and 10 to 290 kDa in Bis-Tris (MES) buffer).
Proteins in this Protein Marker PM2800 are covalently coupled with a blue chromophore except for three reference bands (one green and two red bands at 25 kDa and 75, 310 kDa respectively) when separated on SDS-PAGE in Tris-Glycine buffer.
This SMOBIO ExcelBand 3-color Extra Range Protein Marker PM2800 is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the size of proteins.
This protein marker PM2800 is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25℃), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Under suggested conditions, PM2800 3-color Extra Range Protein Marker resolves 13 major bands in 12% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane.
4°C for 3 months
-20°C for 24 months