WM1000 YesBlot™ Western Marker I, 250 µl/Vial
YesBlot Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in Tris Glycine buffer.
YesBlot Western Marker I performs dual functions.
First, it contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDSPAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size. Second, ten IgG binding proteins can be immuno-detected on film or by CCD imaging.
YesBlot Western Marker I is compatible for chemiluminescent, fluorescent, chromogenic or other detection systems. In addition, YesBlot™ Western Marker I has two reference bands with enhanced intensity (at 30 kDa and 80 kDa).
The Western Blotting YesBlot Western Marker I is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
- 4 prestained proteins for clear visualization during electrophoresis and Western blotting transfer
- 10 IgG-binding proteins for visualization on Western blots
- Wide range size estimation (10-200 kDa)
- Two enhanced bands (30 and 80 kDa)
The YesBlot™ Western Marker I contains recombinant IgG binding proteins, Glycerol, SDS, and tracking dyes in a Tris-HCl buffer.
1.5~2.5 μl per well for two-step Western blot using 1st Ab followed with 2nd Ab conjugated with reporter enzymes.
2.5~5 μl per well for one-step Western blot using 1st Ab conjugated with reporter enzymes.
Apply more for thicker (> 1.5 mm) or larger gel.
4°C for 3 months
-20°C for 24 months